· Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL)
· Immunohistochemistry (Tyner lab)
This is a basic immunohistochemistry protocol. Protocols for immunohistochemistry usr/localy widely, due to the differences between antigens and their recognition by antibody. For success it is crucial to try a number of different techniques and compare the results.
· Treptavidin/Beta-Galactosidase Immunohistochemical Labeling Procedure (KPL)
Immunohistochemical Staining of Floating Thick Sections (10-100 microns) (KPL)
General Protocol for staining thick sections
Immunohistochemical Staining for Formalin-Fixed, Paraffin-Embedded Tissues (LEP)
This staining procedure is for tissues fixed in 10% neutral buffered formalin and embedded in paraffin
Immunohistochemical Stain-Alkaline Phosphatase (APAAP) Technique (Nottingham Pathology Lab)
Immunohistochemisty-Enzymatic Protocol (R&D)
General protocol including details for sample preparation, tissue staining and trouble shooting
Immunohistochemisty-Fluorescence Protocol (R&D)
General protocol
Immunohistochemistry of Mouse Frozen Tissues (eBioscience)
Immunofluorescence- Whole Mount (Karl Clark)
Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically to an antigen (e.g. Luciferase); then a secondary antibody conjugated to a flourochrome or enzyme is bound to the heavy chain constant region of the primary antibody with specificity to the species of origin of the primary (e.g. anti-rabbit conjugated to Rhodamine
Immunohistochemistry on Whole-Mount Embryos (Vesicle Trafficking)
Beta-gal Staining- Whole Mount (Karl Clark)
Beta-gal staining allows identification of embryonic tissues/cells expressing lacZ marker protein by development of pigmented (blue) product in the presence of lacZ enzymatic activity.
X-gal & AP Staining Protocol (Cepko Lab)
Very detailed protocol with background information and several applications.
Histochemical Visualization of Mycoplasma paratuberculosis (KPL)
A semi-permenant mounting medium for immunofluorescence microscopy (House Ear Institute)
A recipe for Moviol,
an inexpensive mounting medium that is easy to prepare.
Microwave Immunohistochemistry(Energy Beam Sciences, Inc.)
Introduction to microwave immunohistochemistry technique and its applicaiton protocol
Antigen Retrieval : for immunohistochemistry (Mike A. Dyer)
Dissociated Cell Staining : immunohistochemistry (Mike A. Dyer)