Isolationofhumancolonicepithelialcells(EC)
1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were confirmed by clinical, radiological, endoscopic, and histological findings. 3.Solid tissues were minced with scissors into small (2-mm3) fragments and incubated for 15 min at room temperature in 100 mM phosphate buffer (pH 7.0) with 6.5 mM DTT to remove mucus contamination.4.Mucosa......閱讀全文
Isolation－of－human－colonic－epithelial－cells－(EC)
1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were confirmed by clini
Isolation－of－Human－Pulmonary－Epithelial－Cells
?Isolation of Human Pulmonary Epithelial Cells????1.?Pulmonary epithelial cells from human lung tissue were isolated.2.?Lungs were perfused with 10
Isolation－of－human－prostatic－epithelial－cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the collagen
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1.?Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2.?All women reported
Isolation－of－human－primary－ovarian－surface－epithelial－cells
1.?Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (References)
Isolation－of－human－primary－gastric－mucosa－epithelial－cells
1.?A piece of gastric mucosa (～3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.?The patients were proven to be no H.
Isolation－of－normal－mammary－epithelial－cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the collagen
Isolation－of－human－atrial－or－ventricular－cells
1.?Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2.?Isolated myocardial tissue w
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.All
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods1.?Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tissue (
Isolation－of－human－uterine－smooth－muscle－cells
1.?Approval to utilize human tissue was given by Hospital's Committee on Research Involving Human Subjects. 2.?Human myometrial and leiomyoma tiss
Isolation－of－human－corneal－endothelial－cells－(HCECs)
Isolation of HCECs1.?The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg
Isolation－and－Culture－of－human－vascular－smooth－muscle－cells
1.?Human vascular smooth muscle cells?(SMCs) were isolated from human saphenous vein.2.?Small moistened pieces of tissue (6 mm2) were placed intima
Isolation－of－colonic－epithelium
The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine.First we excise t
Isolation－of－colonic－epithelium
實驗概要The ?method we use is based on work of Dr. Hazel Cheng, at the University of ?Toronto and works for both colon and the small intestine.First ?we e
Isolation－of－human－multipotent－mesenchymal－stem－cells－from－second
Isolation of human multipotent mesenchymal stem cells from second‐trimester amniotic fluid?Culture of MSC from amniotic fluid1.?Twenty amniotic flui
Isolation－of－stromal－vascular－cells－from－human－adipose－tissue
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs)1.?The human pulmonary arteries were opened to expose the endothelial su
Isolation－and－Culture－of－Multipotent－Stem－Cells－from－Human－Bone－Morrow
Bone marrow contains three types of stem cells:1.?Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
Intestinal－intraepithelial－lymphocytes
Intestinal intraepithelial lymphocytes????Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously associated with gut
Isolation－of－retinal－pigment－epithelial－cell（一）
The pigmented layer of retina or retinal pigment epithelium (RPE) is the pigmented cell layer just outside the neurosensory retina that nourishes
Isolation－of－retinal－pigment－epithelial－cell（二）
15.?This medium is replaced after 1 day with 5% serum-containing RPE medium, and subsequent changes were made every 2 to 3 days.16.?After 3 to 4 weeks
Isolation－of－papillary－cells
實驗概要This protocols provides a general protocol for isolation of papillary cells.實驗步驟Isolation of renal papillary cells1.?For ?isolation of papillary c
Isolation－of－papillary－cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
細胞組織消化常用的幾種酶的選擇
直接從生物體獲取的組織，一般需要將其消化成單個細胞才能進行體外培養。這種直接從離體組織獲得的細胞，更接近于生物體內的生活狀態，且生物性狀尚未發生很大改變，因此在藥物篩選、細胞移植、類器官培養、腫瘤研究等眾多領域備受歡迎。但組織消化過程中常遇到多種問題，例如消化不完全、細胞死亡率高等。如何克服這些問題
Human－FastTrack－mRNA－Isolation
Preparation of Cells1.Prepare or collect between 2x107?cells for each mRNA prep (will yield about 10-20μg of mRNA). If PBMCs from a whole blood sample
表皮細胞的轉染實驗技巧
表皮細胞廣泛遍布于身體，正常的表皮細胞較難轉染，尤其是使用基于脂質體技術的轉染試劑。我們使用電轉(Amaxa)方法轉染正常人的結腸表皮細胞并得到了65%的GFP標記細胞。非常感謝SignaGen,現在我們使用GenJet Ver II可以成功轉染正常人的結腸表皮細胞并且轉染效率顯著提高至75%。
Different－types－of－human－cells
Human primary cells are cells taken from living human beings and cultured.? These cells retain the differentiation of the original cells taken in
Isolation－of－rodent－pancreatic－β－cells
1.?Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2.?Rat islets were isolated from male wistar