PreparationofLuciferinforInVitroandInVivoBioluminescentAssays
實驗概要Reagent for immunoassay, ligand binding assay and ligand receptor assay in which luciferin is covalently bonded to a molecule having biological activity for bonding to a particular biologically active group of a material and method in which the luciferin and biologically active molecule conjugate is added to a material having a group for combination with the biological activity......閱讀全文
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
Preparation of Luciferin forIn Vitro Bioluminescent AssaysMaterials? D-Luciferin Firefly, potassium salt, 1.0 g /vial(Caliper Life Sciences Part Numbe
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
實驗概要Reagent ?for immunoassay, ligand binding assay and ligand receptor assay in ?which luciferin is covalently bonded to a molecule having biological
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
實驗概要Reagent ?for immunoassay, ligand binding assay and ligand receptor assay in ?which luciferin is covalently bonded to a molecule having biological
In－Vivo－Luciferin－Imaging－Procedure
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribut
In－Vitro－prostate－colony－and－sphereforming－assays
1.?Prostates were dissected, minced into small pieces with a steel blade, and digested with 0.8 mg/ml collagenase in 10 ml of primary cell medium/
D螢光素－Protocol－在生物發光檢測中的應用
D-螢光素，螢火蟲螢光素酶的化學發光底物，廣泛用于體外生物發光、體內活體成像。螢螢之光，照亮您的科研之路！?■ Q: D-螢光素的作用原理D-螢光素 (D-Luciferin) 是螢火蟲螢光素酶 (Firefly Luciferase) 的化學發光底物。在ATP 和螢光素酶存在下，螢光素能夠被氧化發
活體成像皮下成瘤實驗操作方法
Materials:MDA-MB-231-Luc and HCT116-Luc cellsMice: 2 subcutaneous HCT116-Luc tumor-bearing female BALB/c nude mice;4 na?ve female BALB/c nude mice;Art
蛋白質磷酸化
Tyrosine Kinase Assay Using Synthetic Peptides?(T. Miller)Small synthetic peptide substrates are especially well suited for applications such as assay
體外熒光法檢測核內體早期動力學
A fluorescence-based?in vitro?assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1?& Silvio O Rizzoli2ABSTRACTEarly endoso
In－vitro－Assessment－of－Metabolic－－in－Suspension－Cryopreserved－Hepatocytes
實驗概要BackgroundThe ?pharmaceutical and biotechnology industry’s goal is to discover ?therapeutic agents that are both safe and effective at treating or
CMFDA－Labeling－of－Platelet
OUTLINECMFDA (5-chloromethylfluorescein diacetate) is a lipophilic tracer that has an enormous advantage over ordinary tracers (e.g. FITC) because it
In－Vitro－Fertilization
When we first started using X. tropicalis,?in vitro?fertilization had an extremely poor efficiency. However, with the careful selection of a mature ma
Agglutination－Assays
Agglutination AssaysREFERENCE:?Lanyi, B., and T. Bergan. Methods in?Microbiology, Vol 10: 93-168.?BACTERIAL AGGLUTINATION:?Bacterial agglutination is
Cellulase－assays
Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the fruit. In cases of p
Cellulase－assays
實驗概要? ? ? ? Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the frui
Carbohydrate－Assays
Carbohydrate AssaysREFERENCE:?Wright and Rebers, Anal. Biochem. 49: 307-319, 1972.OBJECTIVE:?To determine the relative amounts ofLPS carbohydrates pre
Ex－Vivo－Human－Primary－Mesenchymal－Stem－Cells－(MSCs)－Culture
Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs).? MSCs produce adventitial cells in the human bone marrow microenvir
In－vitro－growth－of－seedlings
sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 mi
In－vitro－Sphingomyelinase－Assay
Reagents:Lysis buffer25 mM Tris-HCl, pH 7.45 mM EDTA1 mM ATP20 μg/ml CLAP1 mM PMSFBuffer A10 mM MgCl20.2 M Tris-HCl, pH 7.40.2 % Triton X-100Buffer B0
BrdU－Labeling－Protocol
實驗概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic
In－Vivo－Ubiquitination－Assay－by－Agroinfiltration
The ubiquitination/proteasome system is involved in nearly all plant signaling processes. Many signaling components are degraded by the 26S protea
Measuring－PLD－Activity－In－Vivo
Phospholipase D (PLD) hydrolyzes structural phospholipids like phosphatidylcholine (PC) and phosphatidylethanolamine (PE) into phosphatidic acid (
Microtubule－Binding－Assays
MaterialsSiliconized ultracentrifuge microfuge tubesGTP-depleted microtubules6X SDS loading dye1X SDS loading dyeCoomassie Brilliant Blue R 250 (0.8%
Matrigel－invasion－assays
OverviewMatirgel is considered as basement membrane and generated from EHS sarcoma. Matrigel contains not only basement membrane components (collagens
Cytotoxicity－Assays－Protocol
Cytotoxicity Assays ProtocolCell-mediated cytotoxicity was determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted
活體成像小鼠皮下瘤模型實驗步驟
Luciferin Preparation1.??? Prepare a stock solution of luciferin at 15mg/ml in DPBS. Filter sterilize through a 0.2 um filter.2.??? Prepare enough to
In－vitro－culture－of－embryonic－lungs
In vitro culture of embryonic lungsfrom Hogan LabIsolation of Lung Bud EndodermWhat you need:E11-12 mouse embryosDMEM with 5% fetal bovine serumpetri
Coating－of－Platelets－with－Antibody－in－vitro
OUTLINEAntibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay.?PROTOCOLResuspend 1.6x10^8 of CMFDA-labeled plate
In－Vitro－Protein－Ubiquitination－Assay
Ubiquitination is one of the most important posttranslational modifications in all eukaryote organisms. Ubiquitin-activating enzyme (E1), ubiquiti
體外重組（in－vitro－recombination）
載體與外源DNA分子體外重組時，如何選擇優化連接條件以達到最高的重組率。因此有必要根據影響連接效率的因素綜合考慮連接條件。影響連接效率的因素很多，如反應溫度、插入片段和載體之間的摩爾比、DNA末端性質、反應時間、ATP濃度等。1. 反應溫度是比較重要的影響因素。因為連接酶的最適反應溫度為37℃，